Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 93
... fragments were fractionated by density gradient centrifugation or prepara ... DNA : • The ability to “ walk ” along the eukaryotic chromosome in a way that ... DNA Libraries Bacteriophage λ Analysis and Cloning of Eukaryotic Genomic DNA 9.3.
... fragments were fractionated by density gradient centrifugation or prepara ... DNA : • The ability to “ walk ” along the eukaryotic chromosome in a way that ... DNA Libraries Bacteriophage λ Analysis and Cloning of Eukaryotic Genomic DNA 9.3.
Page 10-52
... DNA with the desired restriction enzyme in 25 μl of the appropriate restriction enzyme buffer . 2. Add 2-10 μCi ( sp . act . 400-3000 Ci / mmole ) of the appropriate [ a- 32P ] dNTP . 3. Add 1 unit of the Klenow fragment of E. coli DNA ...
... DNA with the desired restriction enzyme in 25 μl of the appropriate restriction enzyme buffer . 2. Add 2-10 μCi ( sp . act . 400-3000 Ci / mmole ) of the appropriate [ a- 32P ] dNTP . 3. Add 1 unit of the Klenow fragment of E. coli DNA ...
Page 15-48
... fragments whose lengths are accurately known and whose sizes are approximately equal to that of the target DNA . Such markers can be easily generated by cleaving the original recombi- nant plasmid ... DNA EXCISION OF THE TARGET DNA 15 48.
... fragments whose lengths are accurately known and whose sizes are approximately equal to that of the target DNA . Such markers can be easily generated by cleaving the original recombi- nant plasmid ... DNA EXCISION OF THE TARGET DNA 15 48.
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml