Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-18
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing ... DNA . 13.18 DNA Sequencing FACTORS AFFECTING THE CHOICE BETWEEN RANDOM AND DIRECTED 18.
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing ... DNA . 13.18 DNA Sequencing FACTORS AFFECTING THE CHOICE BETWEEN RANDOM AND DIRECTED 18.
Page 13-102
... DNA sequencing : Chemical methods . Methods Enzymol . 152 : 522 . Anderson , S. , A.T. Bankier , B.G. Barrell , M.H.L. de Bruijn , A.R. Coulson , J. Drouin , I.C. Eperon , D.P. Nierlich ... DNA sequenc- 13.102 DNA Sequencing References 13.
... DNA sequencing : Chemical methods . Methods Enzymol . 152 : 522 . Anderson , S. , A.T. Bankier , B.G. Barrell , M.H.L. de Bruijn , A.R. Coulson , J. Drouin , I.C. Eperon , D.P. Nierlich ... DNA sequenc- 13.102 DNA Sequencing References 13.
Page 13-103
... G.F. Hong , D.F. Hill , and G.B. Petersen . 1982. Nu- cleotide sequence of bacteriophage A DNA . J. Mol . Biol . 162 : 729 . Sanger , F. , J.E. Donelson , A.R. Coulson , H. Kössel , and D. Fischer . 1973. Use of DNA Sequencing 13.103.
... G.F. Hong , D.F. Hill , and G.B. Petersen . 1982. Nu- cleotide sequence of bacteriophage A DNA . J. Mol . Biol . 162 : 729 . Sanger , F. , J.E. Donelson , A.R. Coulson , H. Kössel , and D. Fischer . 1973. Use of DNA Sequencing 13.103.
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml