Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 47
Page 10-11
... DNAase I to generate nicks in the template . Interestingly , the lag is longer when using preparations of DNA that give lower efficiencies of label incorporation . It therefore seems that DNAase I is rate - limiting in the reaction and ...
... DNAase I to generate nicks in the template . Interestingly , the lag is longer when using preparations of DNA that give lower efficiencies of label incorporation . It therefore seems that DNAase I is rate - limiting in the reaction and ...
Page 15-5
... DNAase I. Closed circular plasmid DNA is digested with limited amounts of DNAase I in the presence of a transition metal ion such as Mn * or Co ( Heffron et al . 1978 ) . Under these conditions , DNAase I attacks DNA randomly , cutting ...
... DNAase I. Closed circular plasmid DNA is digested with limited amounts of DNAase I in the presence of a transition metal ion such as Mn * or Co ( Heffron et al . 1978 ) . Under these conditions , DNAase I attacks DNA randomly , cutting ...
Page 15-27
... DNAase I IN THE PRESENCE OF Mn ++ The following protocol is an adaptation of methods described by Anderson ( 1981 ) and Labeit et al . ( 1987 ) . The principle of the method is shown in Figure 15.3 . In brief , a double - stranded ...
... DNAase I IN THE PRESENCE OF Mn ++ The following protocol is an adaptation of methods described by Anderson ( 1981 ) and Labeit et al . ( 1987 ) . The principle of the method is shown in Figure 15.3 . In brief , a double - stranded ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml