Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 78
Page 11-35
... EDTA - Tris ( pH 6.0 ) 2 Add 0.1 mole of EDTA ( free acid m . w . = 292.2 ) to 60 ml of H2O . While stirring the solution , slowly add Tris base ( powder ) until the pH of the solution reaches 6.0 . By this stage , the concentration of ...
... EDTA - Tris ( pH 6.0 ) 2 Add 0.1 mole of EDTA ( free acid m . w . = 292.2 ) to 60 ml of H2O . While stirring the solution , slowly add Tris base ( powder ) until the pH of the solution reaches 6.0 . By this stage , the concentration of ...
Page 13-63
... EDTA / XC / BPB gel - loading buffer formamide 10 ml xylene cyanol FF 10 mg bromophenol blue 10 mg 0.5 м EDTA ( pH 8.0 ) 200 μ . clone no . 1 2 3 4 5 6 T C 0000 G 0000 A T 000 C 0000 G 0000 A 00000 FIGURE 13.9 Microtiter plate ...
... EDTA / XC / BPB gel - loading buffer formamide 10 ml xylene cyanol FF 10 mg bromophenol blue 10 mg 0.5 м EDTA ( pH 8.0 ) 200 μ . clone no . 1 2 3 4 5 6 T C 0000 G 0000 A T 000 C 0000 G 0000 A 00000 FIGURE 13.9 Microtiter plate ...
Page 13-84
... EDTA solution 1 M Piperidine in H2O 1 M Piperidine formate ( pH 2.0 ) Salmon sperm DNA or calf thymus DNA Sequencing gel - loading buffer This solution should be freshly diluted from glacial acetic acid ( 17.4 M ) . 99 % ( Aldrich ...
... EDTA solution 1 M Piperidine in H2O 1 M Piperidine formate ( pH 2.0 ) Salmon sperm DNA or calf thymus DNA Sequencing gel - loading buffer This solution should be freshly diluted from glacial acetic acid ( 17.4 M ) . 99 % ( Aldrich ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml