Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 58
Page 9-16
... ( pH 8.0 ) 0.1 M EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 % SDS It is important that the cells be well - dispersed over the inner surface of the Erlen- meyer flask when the extraction buffer is added . This minimizes the formation ...
... ( pH 8.0 ) 0.1 M EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 % SDS It is important that the cells be well - dispersed over the inner surface of the Erlen- meyer flask when the extraction buffer is added . This minimizes the formation ...
Page 9-20
... ( pH 8.0 ) , 10 mM EDTA ( pH 8.0 ) and six times against 4 liters of 10 mM NaCl , 10 mM Tris Cl ( pH 8.0 ) , 0.5 mM EDTA ( pH 8.0 ) . · 4. Measure the absorbance of the DNA at 260 nm and 280 nm . The ratio of A260 to A280 should be ...
... ( pH 8.0 ) , 10 mM EDTA ( pH 8.0 ) and six times against 4 liters of 10 mM NaCl , 10 mM Tris Cl ( pH 8.0 ) , 0.5 mM EDTA ( pH 8.0 ) . · 4. Measure the absorbance of the DNA at 260 nm and 280 nm . The ratio of A260 to A280 should be ...
Page 13-84
... 8.0 ) 1.5 M sodium acetate ( pH 7.0 ) 1 M ẞ - mercaptoethanol 250 μg / ml ... EDTA ( pH 8.0 ) 100 μg / ml yeast tRNA 1.2 N NaOH 1 mM EDTA This solution should be freshly made by ... Sodium acetate ( pH 5.2 ) Target DNA 13.84 DNA Sequencing 84.
... 8.0 ) 1.5 M sodium acetate ( pH 7.0 ) 1 M ẞ - mercaptoethanol 250 μg / ml ... EDTA ( pH 8.0 ) 100 μg / ml yeast tRNA 1.2 N NaOH 1 mM EDTA This solution should be freshly made by ... Sodium acetate ( pH 5.2 ) Target DNA 13.84 DNA Sequencing 84.
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agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml