Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 30
Page 8-53
... EcoRI sites 4. Ligation to phosphorylated EcoRI linkers and digestion of linkers to yield cohesive termini 5. Size selection of cDNA 6. Ligation to bacteriophage λ arms , packaging of DNA into bacteriophage › particles , and test ...
... EcoRI sites 4. Ligation to phosphorylated EcoRI linkers and digestion of linkers to yield cohesive termini 5. Size selection of cDNA 6. Ligation to bacteriophage λ arms , packaging of DNA into bacteriophage › particles , and test ...
Page 8-66
... EcoRI methylase is inhibited by Mg ++ ions . 2. Remove two aliquots ( 2 μl each ) and place in small ( 0.5 - ml ) microfuge tubes . Number these aliquots 1 and 2. Store at 0 ° C . It is important to use small microfuge tubes to minimize ...
... EcoRI methylase is inhibited by Mg ++ ions . 2. Remove two aliquots ( 2 μl each ) and place in small ( 0.5 - ml ) microfuge tubes . Number these aliquots 1 and 2. Store at 0 ° C . It is important to use small microfuge tubes to minimize ...
Page 8-80
... EcoRI sites within the cDNA inserts is less than 1 site per 4 kb . This problem arises when methylation of EcoRI sites within the double- stranded cDNA is not complete . In this case , it is usually necessary to check the methylation ...
... EcoRI sites within the cDNA inserts is less than 1 site per 4 kb . This problem arises when methylation of EcoRI sites within the double- stranded cDNA is not complete . In this case , it is usually necessary to check the methylation ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml