Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-71
... Figure 8.16 . 6. Add 0.1 volume of 3 м sodium acetate ( pH 5.2 ) and 2 volumes of ethanol to fractions containing ... FIGURE 8.15 FIGURE 8.16 Ligation to Bacteriophage λ Arms Because of the Construction and Analysis of cDNA Libraries 8.71.
... Figure 8.16 . 6. Add 0.1 volume of 3 м sodium acetate ( pH 5.2 ) and 2 volumes of ethanol to fractions containing ... FIGURE 8.15 FIGURE 8.16 Ligation to Bacteriophage λ Arms Because of the Construction and Analysis of cDNA Libraries 8.71.
Page 15-14
... Figure 15.2 ) . ++ • Pancreatic DNAase I : In the presence of Mg ** , DNAase I behaves like an endonuclease that introduces nicks independently into each strand of double - stranded DNA . However , as discussed earlier , in the presence ...
... Figure 15.2 ) . ++ • Pancreatic DNAase I : In the presence of Mg ** , DNAase I behaves like an endonuclease that introduces nicks independently into each strand of double - stranded DNA . However , as discussed earlier , in the presence ...
Page 15-57
... Figure 15.7 ) , two oligonucleotides - the phosphorylated mutagenic oligonucleotide and a universal sequencing primer ( which need not be phosphorylated ) —are mixed in a 10- to 50 - fold molar excess with the single - stranded template ...
... Figure 15.7 ) , two oligonucleotides - the phosphorylated mutagenic oligonucleotide and a universal sequencing primer ( which need not be phosphorylated ) —are mixed in a 10- to 50 - fold molar excess with the single - stranded template ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml