Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-2 of 2
Page 13-16
... GATC GATC U !! TIT i.a. < ! 11 GATC 1 } 1 0 111 Carry out dideoxy - mediated sequencing reactions GATC " ### 11 181 811 1811 WIT Sequence is obtained from the end point of each deletion " FIGURE 13.3A primer 3 primer 1 universal primer ...
... GATC GATC U !! TIT i.a. < ! 11 GATC 1 } 1 0 111 Carry out dideoxy - mediated sequencing reactions GATC " ### 11 181 811 1811 WIT Sequence is obtained from the end point of each deletion " FIGURE 13.3A primer 3 primer 1 universal primer ...
Page 13-79
... GATC G 32 p с CGA T T32A 3 ' GATC [ a - 32P ] dNTP 3 ' 5 Klenow fragment of E. coli DNA polymerase I 32 Isolate target DNA by cleavage with two restriction enzymes , only one of which generates a recessed 3 ' terminus Radiolabel ...
... GATC G 32 p с CGA T T32A 3 ' GATC [ a - 32P ] dNTP 3 ' 5 Klenow fragment of E. coli DNA polymerase I 32 Isolate target DNA by cleavage with two restriction enzymes , only one of which generates a recessed 3 ' terminus Radiolabel ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml