Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 88
Page 9-30
... Incubate for 20 minutes at 37 ° C . 2. Mix 6.5 ml of melted top agar or agarose with each aliquot of infected bacteria and spread on a freshly poured 150 - mm plate of bottom agar . Alternatively , as many as 450,000 bacteriophages may ...
... Incubate for 20 minutes at 37 ° C . 2. Mix 6.5 ml of melted top agar or agarose with each aliquot of infected bacteria and spread on a freshly poured 150 - mm plate of bottom agar . Alternatively , as many as 450,000 bacteriophages may ...
Page 13-40
... incubate the microfuge tubes or microtiter plate for 30 minutes at 30 ° C . Add 1 μl of S1 stop mixture to each of the microfuge tubes or wells , and incubate for 10 minutes at 70 ° C . This inactivates nuclease S1 and any residual ...
... incubate the microfuge tubes or microtiter plate for 30 minutes at 30 ° C . Add 1 μl of S1 stop mixture to each of the microfuge tubes or wells , and incubate for 10 minutes at 70 ° C . This inactivates nuclease S1 and any residual ...
Page 13-86
... Incubate 5 minutes at 20 ° C . Add 50 μl DMS stop solu- tion ( at 0 ° C ) and mix . Add 750 μl of ethanol ( -20 ° C ) and mix . Store for 5 minutes at -70 ° C . Centrifuge 5 minutes at 12,000g at 4 ° C . Remove the supernatant carefully ...
... Incubate 5 minutes at 20 ° C . Add 50 μl DMS stop solu- tion ( at 0 ° C ) and mix . Add 750 μl of ethanol ( -20 ° C ) and mix . Store for 5 minutes at -70 ° C . Centrifuge 5 minutes at 12,000g at 4 ° C . Remove the supernatant carefully ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml