Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-40
... Klenow fragment of E. coli DNA polymer- ase I to synthesize a strand of DNA complementary to the synthetic oligonu- cleotide ( Studencki and Wallace 1984 ; Ullrich et al . 1984b ) ( Figure 11.5 ) . A short primer is hybridized to an ...
... Klenow fragment of E. coli DNA polymer- ase I to synthesize a strand of DNA complementary to the synthetic oligonu- cleotide ( Studencki and Wallace 1984 ; Ullrich et al . 1984b ) ( Figure 11.5 ) . A short primer is hybridized to an ...
Page 13-8
... Klenow enzyme than with Sequenases , perhaps because they decrease still further the already low processivity of the enzyme . In summary , the Klenow fragment of E. coli DNA polymerase I is the enzyme of choice for determining the ...
... Klenow enzyme than with Sequenases , perhaps because they decrease still further the already low processivity of the enzyme . In summary , the Klenow fragment of E. coli DNA polymerase I is the enzyme of choice for determining the ...
Page 13-76
... Klenow fragment is deteriorating or is in limiting concentration Heterogeneity in primer length Possible solutions ... Klenow fragment to 2 units per template Add additional Klenow fragment during the chase ( see note to step 13 , page ...
... Klenow fragment is deteriorating or is in limiting concentration Heterogeneity in primer length Possible solutions ... Klenow fragment to 2 units per template Add additional Klenow fragment during the chase ( see note to step 13 , page ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml