Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 53
Page 12-38
... MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes . 4. To three of the tubes , add 1 × 107 , 5 × 107 , or 2 ...
... MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes . 4. To three of the tubes , add 1 × 107 , 5 × 107 , or 2 ...
Page 13-41
... MgCl2 100 mm dithiothreitol 550 με 100 με 100 μl 250 μ . 5 Weiss units 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) ( optional ) This buffer should be stored in small aliquots at -20 ° C . For definition of Weiss unit , see ...
... MgCl2 100 mm dithiothreitol 550 με 100 με 100 μl 250 μ . 5 Weiss units 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) ( optional ) This buffer should be stored in small aliquots at -20 ° C . For definition of Weiss unit , see ...
Page 14-20
... MgCl2 in the amplification buffer needs to be supplemented with additional MgCl2 in the reverse transcriptase reaction mixture because reverse transcriptase requires higher concentrations of divalent cations than does Taq DNA polymerase ...
... MgCl2 in the amplification buffer needs to be supplemented with additional MgCl2 in the reverse transcriptase reaction mixture because reverse transcriptase requires higher concentrations of divalent cations than does Taq DNA polymerase ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml