Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 3
Page 13-80
... Smal Tth 1111 Tth 1111 BstEll EcoRI Saci 5 ' PSP64CS 5 ' 3 ' Tth 1111 Smal. In PSP64CS , the Hindill site in the polycloning site is downstream from the SP6 promoter . In PSP65CS , the EcoRI site in the polycloning site is downstream ...
... Smal Tth 1111 Tth 1111 BstEll EcoRI Saci 5 ' PSP64CS 5 ' 3 ' Tth 1111 Smal. In PSP64CS , the Hindill site in the polycloning site is downstream from the SP6 promoter . In PSP65CS , the EcoRI site in the polycloning site is downstream ...
Page 13-81
... Smal Tth 1111 TATCATCGCCCTGACTGAGTCGAGCCCGGGCTCGACTTAGTCAGGGTCA ATAGTAGCGGGACTGACTCAGCTCGGGCCCGAGCTGAATCAGTCCCAGT 3 ' 5 ' TATCATCGCCCTGACT ATAGTAGCGGGACTGAC Tth 1111 GAGTCGAGCCCGGGCTCGACT TCAGCTCGGGCCCGAGCTGAA [ a - 32P ] dGTP Klenow ...
... Smal Tth 1111 TATCATCGCCCTGACTGAGTCGAGCCCGGGCTCGACTTAGTCAGGGTCA ATAGTAGCGGGACTGACTCAGCTCGGGCCCGAGCTGAATCAGTCCCAGT 3 ' 5 ' TATCATCGCCCTGACT ATAGTAGCGGGACTGAC Tth 1111 GAGTCGAGCCCGGGCTCGACT TCAGCTCGGGCCCGAGCTGAA [ a - 32P ] dGTP Klenow ...
Page 13-82
... Smal site can be sequenced from both ends . • Because the method is rapid and simple , many different templates can be prepared for sequencing simultaneously . • There is no need to map the positions of restriction sites within the ...
... Smal site can be sequenced from both ends . • Because the method is rapid and simple , many different templates can be prepared for sequencing simultaneously . • There is no need to map the positions of restriction sites within the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml