Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-57
... Store the mixture in aliquots at -70 ° C . Make a stock solution in water . Store in small aliquots at -20 ° C . Use strains C600 ( BNN93 ) for growth and BNN102 ( C600hflA ) for screening of cDNA libraries constructed in bacteriophage ...
... Store the mixture in aliquots at -70 ° C . Make a stock solution in water . Store in small aliquots at -20 ° C . Use strains C600 ( BNN93 ) for growth and BNN102 ( C600hflA ) for screening of cDNA libraries constructed in bacteriophage ...
Page 13-39
... Store the open tube on the bench until the last traces of ethanol have evaporated , and then dissolve the DNA in 60 μl of 1 × exonuclease III buffer . Store the dissolved DNA on ice . 10 × Exonuclease III buffer • 0.66 M Tris Cl ( pH ...
... Store the open tube on the bench until the last traces of ethanol have evaporated , and then dissolve the DNA in 60 μl of 1 × exonuclease III buffer . Store the dissolved DNA on ice . 10 × Exonuclease III buffer • 0.66 M Tris Cl ( pH ...
Page 13-86
... Store for 5 minutes at -70 ° C . Centrifuge 5 minutes at 12,000g at 4 ° C . Remove the supernatant carefully and dispose of it in the appropriate waste bottle . A + G Mix 10 μl H2O 4 μl sonicated DNA 10 μl 32P - labeled DNA Chill to 0 ...
... Store for 5 minutes at -70 ° C . Centrifuge 5 minutes at 12,000g at 4 ° C . Remove the supernatant carefully and dispose of it in the appropriate waste bottle . A + G Mix 10 μl H2O 4 μl sonicated DNA 10 μl 32P - labeled DNA Chill to 0 ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml