Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 27
Page 11-23
... Acrylamide stock acrylamide ( DNA - sequencing grade ) N , N ' - methylenebisacrylamide H2O to 250 ml 190 g 10 g Dissolve the chemicals by heating the solution to 37 ° C . Adjust the volume of the solution to 500 ml . Filter the ...
... Acrylamide stock acrylamide ( DNA - sequencing grade ) N , N ' - methylenebisacrylamide H2O to 250 ml 190 g 10 g Dissolve the chemicals by heating the solution to 37 ° C . Adjust the volume of the solution to 500 ml . Filter the ...
Page 13-47
... Acrylamide solution acrylamide ( DNA - sequencing grade ) N , N ' - methylenebisacrylamide 380 g 20 g distilled H2O to 600 ml Heat the solution to 37 ° C to dissolve the chemicals . Adjust the volume to 1 liter with distilled water ...
... Acrylamide solution acrylamide ( DNA - sequencing grade ) N , N ' - methylenebisacrylamide 380 g 20 g distilled H2O to 600 ml Heat the solution to 37 ° C to dissolve the chemicals . Adjust the volume to 1 liter with distilled water ...
Page 13-48
... Acrylamide / urea top solution 40 % acrylamide solution 75 ml 5x TBE 50 ml urea ( ultrapure ) 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 ...
... Acrylamide / urea top solution 40 % acrylamide solution 75 ml 5x TBE 50 ml urea ( ultrapure ) 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml