Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-30
... agar or agarose with each aliquot of infected bacteria and spread on a freshly poured 150 - mm plate of bottom agar . Alternatively , as many as 450,000 bacteriophages may be mixed with 14 ml of bacteria and plated in 75 ml of top agar ...
... agar or agarose with each aliquot of infected bacteria and spread on a freshly poured 150 - mm plate of bottom agar . Alternatively , as many as 450,000 bacteriophages may be mixed with 14 ml of bacteria and plated in 75 ml of top agar ...
Page 12-23
... agar plate to a nitrocellulose filter . The method works with bacterial colonies of any size , but small colonies ... agar medium , in contact with the bacterial colonies , until it is completely wet . Mark the filter in at least three ...
... agar plate to a nitrocellulose filter . The method works with bacterial colonies of any size , but small colonies ... agar medium , in contact with the bacterial colonies , until it is completely wet . Mark the filter in at least three ...
Page 15-65
... agar ( melted and cooled to 45 ° C ) to each culture , and plate each mixture on a separate YT agar plate . Incubate the plates for 16 hours at 37 ° C to allow plaques to form . If mutagenesis is carried out by the Kunkel method ( see ...
... agar ( melted and cooled to 45 ° C ) to each culture , and plate each mixture on a separate YT agar plate . Incubate the plates for 16 hours at 37 ° C to allow plaques to form . If mutagenesis is carried out by the Kunkel method ( see ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml