Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiii
... Agarose Gel Electrophoresis 6.8 PREPARATION AND EXAMINATION OF AGAROSE GELS 6.9 Preparation of an Agarose Gel 6.9 Minigels 6.14 Staining DNA in Agarose Gels 6.15 Decontamination of Ethidium Bromide Solutions 6.16 DECONTAMINATION OF ...
... Agarose Gel Electrophoresis 6.8 PREPARATION AND EXAMINATION OF AGAROSE GELS 6.9 Preparation of an Agarose Gel 6.9 Minigels 6.14 Staining DNA in Agarose Gels 6.15 Decontamination of Ethidium Bromide Solutions 6.16 DECONTAMINATION OF ...
Page xviii
... AGAROSE GEL ELECTROPHORESIS 9.32 TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE ...
... AGAROSE GEL ELECTROPHORESIS 9.32 TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE ...
Page 10-10
... agarose . Thus , DNA samples eluted from agarose gels should be carefully purified as described in Chapter 6 , pages 6.32-6.35 , before they are used in nick - translation reactions ( see also alternative method for nick transla- tion ...
... agarose . Thus , DNA samples eluted from agarose gels should be carefully purified as described in Chapter 6 , pages 6.32-6.35 , before they are used in nick - translation reactions ( see also alternative method for nick transla- tion ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml