Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiii
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE CONCENTRATION 6.4 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE CONCENTRATION 6.4 ...
Page 9-34
A Laboratory Manual Joseph Sambrook, Tom Maniatis. TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS There are three methods to transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS There are three methods to transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) ...
Page 9-41
... AGAROSE GEL TO TWO NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the ...
... AGAROSE GEL TO TWO NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml