Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 76
Page 8-57
... aliquots at -70 ° C . Make a stock solution in water . Store in small aliquots at -20 ° C . Use strains C600 ( BNN93 ) for growth and BNN102 ( C600hflA ) for screening of cDNA libraries constructed in bacteriophage Agt10 . Use strain ...
... aliquots at -70 ° C . Make a stock solution in water . Store in small aliquots at -20 ° C . Use strains C600 ( BNN93 ) for growth and BNN102 ( C600hflA ) for screening of cDNA libraries constructed in bacteriophage Agt10 . Use strain ...
Page 8-66
... aliquots ( 2 μl each ) from the large - scale reaction and place in small microfuge tubes . Number these aliquots 3 and 4 . 5. To all four aliquots , add 100 ng of a plasmid such as Xf3 or pBR322 that has been digested to completion ...
... aliquots ( 2 μl each ) from the large - scale reaction and place in small microfuge tubes . Number these aliquots 3 and 4 . 5. To all four aliquots , add 100 ng of a plasmid such as Xf3 or pBR322 that has been digested to completion ...
Page 15-65
... aliquots of the undiluted reaction mixture and of each dilution of the reaction mixture with 200 - μl aliquots of competent TG1 cells ( prepared as described in Chapter 1 , pages 1.76-1.81 ) . c . Store the mixtures on ice for 30 ...
... aliquots of the undiluted reaction mixture and of each dilution of the reaction mixture with 200 - μl aliquots of competent TG1 cells ( prepared as described in Chapter 1 , pages 1.76-1.81 ) . c . Store the mixtures on ice for 30 ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml