Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 92
Page 8-27
... allow the sequences at the 5 ' terminus of the mRNA ( which are often lost during conventional cloning procedures ) to be cloned . Its major disadvantage , however , is that it is at least ten times less efficient than double - stranded ...
... allow the sequences at the 5 ' terminus of the mRNA ( which are often lost during conventional cloning procedures ) to be cloned . Its major disadvantage , however , is that it is at least ten times less efficient than double - stranded ...
Page 8-70
... allow the cDNA to enter the gel matrix . Wash the microfuge tube used to store the cDNA with 50 μl of TE ( pH 7.6 ) , and apply this to the column . Fill the bubble tubing with TE ( pH 7.6 ) containing 0.1 M NaCl . Important : Do not allow ...
... allow the cDNA to enter the gel matrix . Wash the microfuge tube used to store the cDNA with 50 μl of TE ( pH 7.6 ) , and apply this to the column . Fill the bubble tubing with TE ( pH 7.6 ) containing 0.1 M NaCl . Important : Do not allow ...
Page 9-22
... Allow the powders to spread over the surface of the lysis solution , and then shake the beakers to submerge the material . When all the material is in solution , transfer the solutions to centrifuge tubes . 3. Close the tops of the ...
... Allow the powders to spread over the surface of the lysis solution , and then shake the beakers to submerge the material . When all the material is in solution , transfer the solutions to centrifuge tubes . 3. Close the tops of the ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml