Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 27
Page 8-49
... amino acid sequence of the protein of interest . Because of the degeneracy of the genetic code , it is very unlikely that a given sequence of amino acids will be specified by a predictable single oligonucleotide of defined sequence ...
... amino acid sequence of the protein of interest . Because of the degeneracy of the genetic code , it is very unlikely that a given sequence of amino acids will be specified by a predictable single oligonucleotide of defined sequence ...
Page 11-5
... amino acids have been used as hybridization probes to isolate both cDNA ... acid sequence determined by the sequencing of small quantities of highly ... amino acids . For example : ALA TRP LYS Amino acid sequence : VAL ASN PHE TYR ...
... amino acids have been used as hybridization probes to isolate both cDNA ... acid sequence determined by the sequencing of small quantities of highly ... amino acids . For example : ALA TRP LYS Amino acid sequence : VAL ASN PHE TYR ...
Page 11-9
... amino acid sequence information that is available . The following guidelines should be used to design degenerate pools that correspond to each separate tract of amino acids . 1. The entire available protein sequence should be examined ...
... amino acid sequence information that is available . The following guidelines should be used to design degenerate pools that correspond to each separate tract of amino acids . 1. The entire available protein sequence should be examined ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml