Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-61
... amount of [ a - 32P ] dCTP ( 10 μCi total ) in the large - scale reaction described above and a much greater amount ( 100 μCi total ) of radiolabeled dNTP in the subsequent reaction to synthesize the second strand of cDNA . This ...
... amount of [ a - 32P ] dCTP ( 10 μCi total ) in the large - scale reaction described above and a much greater amount ( 100 μCi total ) of radiolabeled dNTP in the subsequent reaction to synthesize the second strand of cDNA . This ...
Page 8-73
... amount of bacteriophage arms is ligated to varying amounts of cDNA . The aim is to determine the amount of cDNA that yields at least 5 × 10 ° recombinant bacteriophages ( i.e. , a mammalian cDNA library of reasonable size ) . The ...
... amount of bacteriophage arms is ligated to varying amounts of cDNA . The aim is to determine the amount of cDNA that yields at least 5 × 10 ° recombinant bacteriophages ( i.e. , a mammalian cDNA library of reasonable size ) . The ...
Page 10-9
... amount of the unlabeled dNTP . Most commercial suppliers sell [ a - 32P ] dNTPs in a concentrated aqueous solution ... amount of DNAase I in the reaction . The aim is to establish conditions that will result in incorporation of about 30 ...
... amount of the unlabeled dNTP . Most commercial suppliers sell [ a - 32P ] dNTPs in a concentrated aqueous solution ... amount of DNAase I in the reaction . The aim is to establish conditions that will result in incorporation of about 30 ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml