Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 14
Page 12-38
... ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes . 4. To three of the tubes , add 1 ...
... ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes . 4. To three of the tubes , add 1 ...
Page 15-45
... ampicillin and kanamycin , each at a concentration of 100 μg / ml . Use one half of the bacterial suspension to inoculate a 500 - ml culture ( LB medium containing ampicillin and kanamycin ) . Grow the culture to saturation at 37 ° C ...
... ampicillin and kanamycin , each at a concentration of 100 μg / ml . Use one half of the bacterial suspension to inoculate a 500 - ml culture ( LB medium containing ampicillin and kanamycin ) . Grow the culture to saturation at 37 ° C ...
Page 15-46
... ampicillin ( i.e. , the bacterial plates used to select transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at −20 ° C . 11. From the ...
... ampicillin ( i.e. , the bacterial plates used to select transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at −20 ° C . 11. From the ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml