Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 14-5
A Laboratory Manual Joseph Sambrook, Tom Maniatis. APPLICATIONS OF PCR AMPLIFICATION Although a relatively new technique , PCR amplification has already found extensive application in the diagnosis of genetic disorders ( Wong et al ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. APPLICATIONS OF PCR AMPLIFICATION Although a relatively new technique , PCR amplification has already found extensive application in the diagnosis of genetic disorders ( Wong et al ...
Page 14-10
... amplification , one primer is essentially depleted and the final stages of the amplification reaction therefore generate copies of only one strand of the original DNA ( Gyllen- sten and Erlich 1988 ; Mihovilovic and Lee 1989 ) ...
... amplification , one primer is essentially depleted and the final stages of the amplification reaction therefore generate copies of only one strand of the original DNA ( Gyllen- sten and Erlich 1988 ; Mihovilovic and Lee 1989 ) ...
Page 14-28
... Amplification In this method ( Gyllensten and Erlich 1988 ; Mihovilovic and Lee 1989 ) , amplification reactions are set up that contain vastly different molar ratios of the two oligonucleotide primers . During the first 10-15 cycles of ...
... Amplification In this method ( Gyllensten and Erlich 1988 ; Mihovilovic and Lee 1989 ) , amplification reactions are set up that contain vastly different molar ratios of the two oligonucleotide primers . During the first 10-15 cycles of ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml