Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 72
Page 8-50
... antibody . It is essential that the antibody efficiently recognize the denatured protein ( i.e. , it should produce strong signals on western blots ) . Screening is made easier and more sensitive if , in addition , the antibody is ...
... antibody . It is essential that the antibody efficiently recognize the denatured protein ( i.e. , it should produce strong signals on western blots ) . Screening is made easier and more sensitive if , in addition , the antibody is ...
Page 12-14
... antibody bound to its antigen on nitrocellulose filters are : . 125 I - labeled antibodies reactive with species - specific determinants on the primary antibodies ( Helfman et al . 1983 ) . • Protein A of Staphylococcus aureus ...
... antibody bound to its antigen on nitrocellulose filters are : . 125 I - labeled antibodies reactive with species - specific determinants on the primary antibodies ( Helfman et al . 1983 ) . • Protein A of Staphylococcus aureus ...
Page 12-19
... antibody diluted in blocking buffer ( 7.5 ml for each 82 - mm filter ; 15 ml for each 138 - mm filter ) . Use the highest dilution of antibody that gives acceptable background yet still allows detection of 50-100 pg of denatured antigen ...
... antibody diluted in blocking buffer ( 7.5 ml for each 82 - mm filter ; 15 ml for each 138 - mm filter ) . Use the highest dilution of antibody that gives acceptable background yet still allows detection of 50-100 pg of denatured antigen ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml