Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 88
Page 8-5
... approximately 500 bases to more than 5 kb ( Figure 8.2 ) . The bulk of the radioactivity should lie between 1.5 kb ... approximately 700 nu- cleotides to more than 8000 nucleotides in length . Second - strand cDNA made by self - priming ...
... approximately 500 bases to more than 5 kb ( Figure 8.2 ) . The bulk of the radioactivity should lie between 1.5 kb ... approximately 700 nu- cleotides to more than 8000 nucleotides in length . Second - strand cDNA made by self - priming ...
Page 11-32
... approximately 0.5 cm with developing buffer ( 0.5 м ammonium bicarbonate ) . Cover the jar and allow the chromatograph to develop until the solvent front has migrated approximately 4–5 inches . c . Wrap the strip of polyethyleneimine ...
... approximately 0.5 cm with developing buffer ( 0.5 м ammonium bicarbonate ) . Cover the jar and allow the chromatograph to develop until the solvent front has migrated approximately 4–5 inches . c . Wrap the strip of polyethyleneimine ...
Page 15-45
... approximately 5 ml of LB medium containing ampicillin and kanamycin , each at a concentration of 100 μg / ml . Use a bent glass rod and a pasteur pipette to scrape and squirt the colonies from the surfaces of the plates . Pool the ...
... approximately 5 ml of LB medium containing ampicillin and kanamycin , each at a concentration of 100 μg / ml . Use a bent glass rod and a pasteur pipette to scrape and squirt the colonies from the surfaces of the plates . Pool the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml