Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 41
Page 9-18
... aqueous phases . 5. With a wide - bore pipette ( 0.3 - cm - diameter orifice ) , transfer the viscous aqueous phase to a clean centrifuge tube and repeat the extraction with phenol twice . When transferring the aqueous phase , it is ...
... aqueous phases . 5. With a wide - bore pipette ( 0.3 - cm - diameter orifice ) , transfer the viscous aqueous phase to a clean centrifuge tube and repeat the extraction with phenol twice . When transferring the aqueous phase , it is ...
Page 9-47
... aqueous solution . However , it has been found that the kinetics of hybridization in 80 % formamide are approximately four times slower than in aqueous solution ( Casey and Davidson 1977 ) . Assuming a linear relationship between the ...
... aqueous solution . However , it has been found that the kinetics of hybridization in 80 % formamide are approximately four times slower than in aqueous solution ( Casey and Davidson 1977 ) . Assuming a linear relationship between the ...
Page 15-42
... aqueous phases by centrifugation at 12,000g for 3 minutes at 4 ° C in a microfuge , and transfer the aqueous phase to a fresh microfuge tube . Add 0.5 volume of 5 м ammonium acetate and 2.5 volumes of ethanol to the aqueous phase , and ...
... aqueous phases by centrifugation at 12,000g for 3 minutes at 4 ° C in a microfuge , and transfer the aqueous phase to a fresh microfuge tube . Add 0.5 volume of 5 м ammonium acetate and 2.5 volumes of ethanol to the aqueous phase , and ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml