Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 38
Page 8-73
... arms to cDNA such that only 5 % of the resulting bacteriophages are recombi- nant . If dephosphorylated arms are used , the background of nonrecombi- nant bacteriophages is effectively suppressed , and it is therefore impossible to ...
... arms to cDNA such that only 5 % of the resulting bacteriophages are recombi- nant . If dephosphorylated arms are used , the background of nonrecombi- nant bacteriophages is effectively suppressed , and it is therefore impossible to ...
Page 9-8
... arms that can be efficiently ligated to foreign DNA and does not yield a high proportion of nonrecombinant bacteriophages . The arms of many vectors are available from commercial sources , and it may be cost - effective for those ...
... arms that can be efficiently ligated to foreign DNA and does not yield a high proportion of nonrecombinant bacteriophages . The arms of many vectors are available from commercial sources , and it may be cost - effective for those ...
Page 9-12
... arms may be purified from the central stuffer fragment by density gradient centrifugation or gel electrophoresis . Al- ternatively , highly purified arms may be purchased from commercial vendors . • Fragments of genomic DNA that have ...
... arms may be purified from the central stuffer fragment by density gradient centrifugation or gel electrophoresis . Al- ternatively , highly purified arms may be purchased from commercial vendors . • Fragments of genomic DNA that have ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml