Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 89
Page 15-45
... bacterial suspension obtained from each of the four plates , and recover the bacterial cells by centrifugation at 5000g for 10 minutes at 4 ° C . Remove the supernatant medium . 6. Resuspend the bacterial suspension in 5 ml of LB medium ...
... bacterial suspension obtained from each of the four plates , and recover the bacterial cells by centrifugation at 5000g for 10 minutes at 4 ° C . Remove the supernatant medium . 6. Resuspend the bacterial suspension in 5 ml of LB medium ...
Page 15-46
... bacterial plates used to select transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at −20 ° C . 11. From the numbers of colonies ...
... bacterial plates used to select transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at −20 ° C . 11. From the numbers of colonies ...
Page 1-32
... bacterial conjugation , 1.5 open reading frame vectors for bacterial expression , 17.4 , 17.7 PMC9 , genetic marker in bacteriophage A host strain , 2.57 recombinant , detection in bacterial colonies a - complementation of defective ß ...
... bacterial conjugation , 1.5 open reading frame vectors for bacterial expression , 17.4 , 17.7 PMC9 , genetic marker in bacteriophage A host strain , 2.57 recombinant , detection in bacterial colonies a - complementation of defective ß ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml