Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 32
Page viii
... BACTERIOPHAGE & VECTORS 2.55 Restriction and Modification 2.55 Amber Suppressors 2.55 Recombination Systems 2.55 ... PARTICLES 2.77 GLYCEROL STEP GRADIENT 2.78 EQUILIBRIUM CENTRIFUGATION IN CESIUM CHLORIDE 2.79 EXTRACTION OF BACTERIOPHAGE ...
... BACTERIOPHAGE & VECTORS 2.55 Restriction and Modification 2.55 Amber Suppressors 2.55 Recombination Systems 2.55 ... PARTICLES 2.77 GLYCEROL STEP GRADIENT 2.78 EQUILIBRIUM CENTRIFUGATION IN CESIUM CHLORIDE 2.79 EXTRACTION OF BACTERIOPHAGE ...
Page 8-74
... bacteriophage à particles ( see Chapter 2 , page 2.104 ) . Because the size of the final cDNA library depends on the efficiency with which recombinant bacteriophage A genomes are packaged into infectious bacteriophage particles , it is ...
... bacteriophage à particles ( see Chapter 2 , page 2.104 ) . Because the size of the final cDNA library depends on the efficiency with which recombinant bacteriophage A genomes are packaged into infectious bacteriophage particles , it is ...
Page 8-75
... bacteria in 10 mM MgSO , as described in Chapter 2 , page 2.60 . Measure the number of infectious bacteriophage particles in the packaging mixtures as described on page 2.61 , using 3 ml of LB top agarose containing 40 μl of a stock ...
... bacteria in 10 mM MgSO , as described in Chapter 2 , page 2.60 . Measure the number of infectious bacteriophage particles in the packaging mixtures as described on page 2.61 , using 3 ml of LB top agarose containing 40 μl of a stock ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml