Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-17
... bases , a " neutral " base can be used that pairs adequately with all four conventional bases . The deleterious effect of mismatching is greatly reduced because the base pairs formed between the neutral base and its conventional ...
... bases , a " neutral " base can be used that pairs adequately with all four conventional bases . The deleterious effect of mismatching is greatly reduced because the base pairs formed between the neutral base and its conventional ...
Page 13-11
... base or type of base . This generates five populations of radiolabeled molecules that extend from a common point ( the radiolabeled terminus ) to the site of chemical cleavage . Each population consists of a mixture of molecules whose ...
... base or type of base . This generates five populations of radiolabeled molecules that extend from a common point ( the radiolabeled terminus ) to the site of chemical cleavage . Each population consists of a mixture of molecules whose ...
Page 13-100
... base specificity . Cleavage is consistent with base specificity every- where else in the sequencing pattern . 13. Difficulty in identifying the band corre- sponding to chemical cleavage of the ulti- mate 5 ' nucleotide ( 32P - labeled ...
... base specificity . Cleavage is consistent with base specificity every- where else in the sequencing pattern . 13. Difficulty in identifying the band corre- sponding to chemical cleavage of the ulti- mate 5 ' nucleotide ( 32P - labeled ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml