Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 90
Page 10-54
... buffer , it is necessary to carry out the restriction endonuclease digestion and end - labeling in two separate steps . In this case , cleave the DNA in the appropriate restriction enzyme buffer , remove the restriction enzyme by ...
... buffer , it is necessary to carry out the restriction endonuclease digestion and end - labeling in two separate steps . In this case , cleave the DNA in the appropriate restriction enzyme buffer , remove the restriction enzyme by ...
Page 12-18
... buffer gently for a further 30 minutes at room temperature . If necessary , the filters may be removed from the buffer at this stage , wrapped in Saran Wrap , and stored for up to 24 hours at 4 ° C . 11. Using blunt - ended forceps ...
... buffer gently for a further 30 minutes at room temperature . If necessary , the filters may be removed from the buffer at this stage , wrapped in Saran Wrap , and stored for up to 24 hours at 4 ° C . 11. Using blunt - ended forceps ...
Page 1-45
... buffer , 10.33 promoters . See Promoters termination and polyadenylation sig- nals , 16.5-16.7 upstream mouse ... buffer , 1.78 morphological , by oncogenes , 16.9 test ligations and transformations , 1.62 TFB buffer , 1.78 Translation ...
... buffer , 10.33 promoters . See Promoters termination and polyadenylation sig- nals , 16.5-16.7 upstream mouse ... buffer , 1.78 morphological , by oncogenes , 16.9 test ligations and transformations , 1.62 TFB buffer , 1.78 Translation ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml