Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xvi
... cDNA Cloning 8.3 PREPARATION OF mRNA FOR CDNA CLONING 8.3 Source of the mRNA 8.3 Integrity of the mRNA 8.4 8.6 Abundant mRNAs Low - abundance mRNAs 8.6 Methods of Enrichment 8.6 FRACTIONATION OF mRNA BY SIZE 8.8 FRACTIONATION OF CDNA ...
... cDNA Cloning 8.3 PREPARATION OF mRNA FOR CDNA CLONING 8.3 Source of the mRNA 8.3 Integrity of the mRNA 8.4 8.6 Abundant mRNAs Low - abundance mRNAs 8.6 Methods of Enrichment 8.6 FRACTIONATION OF mRNA BY SIZE 8.8 FRACTIONATION OF CDNA ...
Page 8-27
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Alternative Methods of Cloning cDNA mRNA - CDNA CLONING Several groups have obtained cDNA clones by transformation of E. coli with mRNA : cDNA hybrids that have been joined to plasmid ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Alternative Methods of Cloning cDNA mRNA - CDNA CLONING Several groups have obtained cDNA clones by transformation of E. coli with mRNA : cDNA hybrids that have been joined to plasmid ...
Page 8-31
... cDNA to the vector , the second strand of cDNA is synthesized by a conventional extension reaction using the homopolymeric tract of dG residues as a primer . In a similar protocol ... cDNA Libraries 8.31 CDNA CLONING WITH PRIMER-ADAPTERS 8.
... cDNA to the vector , the second strand of cDNA is synthesized by a conventional extension reaction using the homopolymeric tract of dG residues as a primer . In a similar protocol ... cDNA Libraries 8.31 CDNA CLONING WITH PRIMER-ADAPTERS 8.
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml