Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxvi
... Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and Amplification of ...
... Cells Expression of Proteins 16.3 EXPRESSION OF PROTEINS FROM CLONED GENES FUNCTIONAL COMPONENTS OF MAMMALIAN EXPRESSION VECTORS 16.5 Prokaryotic Plasmid Sequences That Facilitate the Construction , Propagation , and Amplification of ...
Page 9-16
... cell samples Cells growing in monolayers : Wash the monolayers twice with ice - cold Tris - buffered saline ( TBS ; see Appendix B ) . Using a policeman , scrape the cells into approximately 0.5 ml of TBS . Transfer the cell suspension ...
... cell samples Cells growing in monolayers : Wash the monolayers twice with ice - cold Tris - buffered saline ( TBS ; see Appendix B ) . Using a policeman , scrape the cells into approximately 0.5 ml of TBS . Transfer the cell suspension ...
Page 10-38
... cells . The identifi- cation of cDNA clones may be accomplished by a number of methods , all of which are capable of detecting differences in gene expression between closely related types of cells . This section presents methods to ...
... cells . The identifi- cation of cDNA clones may be accomplished by a number of methods , all of which are capable of detecting differences in gene expression between closely related types of cells . This section presents methods to ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml