Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 82
Page 9-16
... centrifuge tube . Recover the cells by centrifugation at 1500g for 10 minutes at 4 ° C . Resuspend the cells in 5-10 volumes of ice - cold TBS and repeat the centrifugation . Resuspend the cells in TE ( pH 8.0 ) at a concentration of 5 ...
... centrifuge tube . Recover the cells by centrifugation at 1500g for 10 minutes at 4 ° C . Resuspend the cells in 5-10 volumes of ice - cold TBS and repeat the centrifugation . Resuspend the cells in TE ( pH 8.0 ) at a concentration of 5 ...
Page 14-23
... centrifuge , and take off the retentate cup . Add 0.75 ml of H2O to the sample reservoir , replace the retentate cup , and recentrifuge as described in step 4 . 6. Repeat step 5 twice more . At the end of the fourth centrifugation , the ...
... centrifuge , and take off the retentate cup . Add 0.75 ml of H2O to the sample reservoir , replace the retentate cup , and recentrifuge as described in step 4 . 6. Repeat step 5 twice more . At the end of the fourth centrifugation , the ...
Page 15-37
... centrifugation at 5000g for 5 minutes at 4 ° C . Carefully decant the ethanol , and wash the pellet with 3 ml of 70 % ethanol at room temperature . Again , recover the DNA by centrifugation , and remove the 70 % ethanol by careful ...
... centrifugation at 5000g for 5 minutes at 4 ° C . Carefully decant the ethanol , and wash the pellet with 3 ml of 70 % ethanol at room temperature . Again , recover the DNA by centrifugation , and remove the 70 % ethanol by careful ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml