Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-35
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . 8. Remove as much of the ethanol as possible by gentle aspiration , and leave the open tube on the bench for a few minutes to allow the last visible traces of ethanol to ...
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . 8. Remove as much of the ethanol as possible by gentle aspiration , and leave the open tube on the bench for a few minutes to allow the last visible traces of ethanol to ...
Page 13-72
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . 7. Carefully remove the supernatants by aspiration , and add 1 ml of 70 % ethanol to each tube . Vortex the tube briefly , and then recentrifuge at 12,000g for 5 minutes ...
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . 7. Carefully remove the supernatants by aspiration , and add 1 ml of 70 % ethanol to each tube . Vortex the tube briefly , and then recentrifuge at 12,000g for 5 minutes ...
Page 15-11
... centrifugation at 12,000g for 3 minutes at 4 ° C in a microfuge . d . Carefully transfer the aqueous phase to the top of a spun column of Sephadex G - 75 . Separate the plasmid DNA from the remnants of linkers by spun - column ...
... centrifugation at 12,000g for 3 minutes at 4 ° C in a microfuge . d . Carefully transfer the aqueous phase to the top of a spun column of Sephadex G - 75 . Separate the plasmid DNA from the remnants of linkers by spun - column ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml