Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 61
Page 13-88
... cleavage reactions are carried out on one day and the gels are run on the following day . However , with practice , it is possible to complete both cleavage and electrophoresis in a single working day , if a sequencing gel is prepared ...
... cleavage reactions are carried out on one day and the gels are run on the following day . However , with practice , it is possible to complete both cleavage and electrophoresis in a single working day , if a sequencing gel is prepared ...
Page 13-99
... cleavage . Purify restriction enzyme , phosphatase , ki- nase , or terminal transferase . After labeling ragged ends , cleave with re- striction enzymes that will give end seg- ments less than 100 bp long , denature , and fractionate on ...
... cleavage . Purify restriction enzyme , phosphatase , ki- nase , or terminal transferase . After labeling ragged ends , cleave with re- striction enzymes that will give end seg- ments less than 100 bp long , denature , and fractionate on ...
Page 13-100
... cleavage reactions that do not normally share base specificity . Cleavage is consistent with base specificity every- where else in the sequencing pattern . 13. Difficulty in identifying the band corre- sponding to chemical cleavage of ...
... cleavage reactions that do not normally share base specificity . Cleavage is consistent with base specificity every- where else in the sequencing pattern . 13. Difficulty in identifying the band corre- sponding to chemical cleavage of ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml