Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 66
Page 8-24
... cleaved vector with a phosphatase before ligation to cDNA . If the double - stranded cDNA contains one or more recognition sites for the restriction enzyme , it will be cleaved and subsequently cloned as two or more DNA fragments ...
... cleaved vector with a phosphatase before ligation to cDNA . If the double - stranded cDNA contains one or more recognition sites for the restriction enzyme , it will be cleaved and subsequently cloned as two or more DNA fragments ...
Page 13-99
... cleaved at adenines carry too many ring - opened 7- methylguanines and are heterogeneous in charge . Sample not evenly distributed in sample well . Nested end - labeled cleavage products be- come long enough to fold into stable hair ...
... cleaved at adenines carry too many ring - opened 7- methylguanines and are heterogeneous in charge . Sample not evenly distributed in sample well . Nested end - labeled cleavage products be- come long enough to fold into stable hair ...
Page 15-7
... cleave DNA frequently . Enzymes that generate blunt ends ( FnuDII , AluI , RsaI , and Hae III ) are particularly useful ... cleaved more than once by frequent- ly cutting restriction enzymes . Despite their problems , both of the methods ...
... cleave DNA frequently . Enzymes that generate blunt ends ( FnuDII , AluI , RsaI , and Hae III ) are particularly useful ... cleaved more than once by frequent- ly cutting restriction enzymes . Despite their problems , both of the methods ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml