Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-7
... clones required , P = the probability desired ( usually 0.99 ) , and 1 / n = the fractional proportion of the total ... clones . During the last few years , the efficiency with which cDNA can be synthesized and cloned has increased to ...
... clones required , P = the probability desired ( usually 0.99 ) , and 1 / n = the fractional proportion of the total ... clones . During the last few years , the efficiency with which cDNA can be synthesized and cloned has increased to ...
Page 8-46
... cloning projects today are aimed at isolating cDNAs corresponding to rare mRNAs and therefore require screening of large numbers of recombinant clones . This can be carried out effectively with only two types of reagents : antibodies ...
... cloning projects today are aimed at isolating cDNAs corresponding to rare mRNAs and therefore require screening of large numbers of recombinant clones . This can be carried out effectively with only two types of reagents : antibodies ...
Page 11-5
... cloned copies of the gene of interest can be readily isolated from cDNA or genomic DNA libraries . However , it is important to realize that not all strongly hybridizing clones will necessarily contain sequences of the gene of interest ...
... cloned copies of the gene of interest can be readily isolated from cDNA or genomic DNA libraries . However , it is important to realize that not all strongly hybridizing clones will necessarily contain sequences of the gene of interest ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml