Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 29
Page 11-9
... codons . Regions rich in amino acids with six codon choices ( Leu , Ser , and Arg ) should be avoided . The number of codons that encode each amino acid are given below ( see also Figure 11.1 ) . 1 2 3 4 6 Met Phe Ile Val Leu Trp Tyr ...
... codons . Regions rich in amino acids with six codon choices ( Leu , Ser , and Arg ) should be avoided . The number of codons that encode each amino acid are given below ( see also Figure 11.1 ) . 1 2 3 4 6 Met Phe Ile Val Leu Trp Tyr ...
Page 11-11
... codons that differ only in the third position , so that at least two of the three nucleotides of each codon are guaranteed to be perfectly matched . Hybridization can therefore be carried out under comparatively stringent conditions ...
... codons that differ only in the third position , so that at least two of the three nucleotides of each codon are guaranteed to be perfectly matched . Hybridization can therefore be carried out under comparatively stringent conditions ...
Page 11-14
... codon choices that generate the sequence CpG between codons . This dinucleotide is significantly underrepresented in mammalian DNA ( Bird 1980 ) , and CpG occurs in intercodons at about one half of the expected frequency ( Lathe 1985 ) ...
... codon choices that generate the sequence CpG between codons . This dinucleotide is significantly underrepresented in mammalian DNA ( Bird 1980 ) , and CpG occurs in intercodons at about one half of the expected frequency ( Lathe 1985 ) ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml