Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-23
... termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated ... cohesive termini compatible with those of the linker . Double - stranded cDNA molecules containing the synthetic cohesive ...
... termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated ... cohesive termini compatible with those of the linker . Double - stranded cDNA molecules containing the synthetic cohesive ...
Page 8-24
... cohesive termini will of course ligate to each other as well as to the vector DNA . It is therefore important to ... termini of the cDNA and cleaved with the appropriate restriction enzyme to generate molecules with cohesive termini ...
... cohesive termini will of course ligate to each other as well as to the vector DNA . It is therefore important to ... termini of the cDNA and cleaved with the appropriate restriction enzyme to generate molecules with cohesive termini ...
Page 15-3
... cohesive termini , ligation can be carried out directly . However , if incompatible cohesive termini are generated , the Klenow fragment of E. coli DNA polymerase I , bacteriophage T4 DNA polymerase , nuclease S1 , or mung - bean ...
... cohesive termini , ligation can be carried out directly . However , if incompatible cohesive termini are generated , the Klenow fragment of E. coli DNA polymerase I , bacteriophage T4 DNA polymerase , nuclease S1 , or mung - bean ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml