Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 54
Page 12-21
A Laboratory Manual Joseph Sambrook, Tom Maniatis. SCREENING BACTERIAL COLONIES Preparation of Colonies for Screening METHOD 1 This procedure ( Hanahan and Meselson 1980 ) is used when you know in advance that you will need to screen ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. SCREENING BACTERIAL COLONIES Preparation of Colonies for Screening METHOD 1 This procedure ( Hanahan and Meselson 1980 ) is used when you know in advance that you will need to screen ...
Page 12-23
... colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results : They produce sharper signals and smear less than larger colonies . As many as 2 × 10 colonies per 150 - mm plate can be screened by this technique . Note ...
... colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results : They produce sharper signals and smear less than larger colonies . As many as 2 × 10 colonies per 150 - mm plate can be screened by this technique . Note ...
Page 15-72
... colonies almost certainly contain a mixture of wild - type and mutated molecules , it is necessary to carry out a second round of transformation and screening as outlined below . Note that simple restreaking of positive colonies does ...
... colonies almost certainly contain a mixture of wild - type and mutated molecules , it is necessary to carry out a second round of transformation and screening as outlined below . Note that simple restreaking of positive colonies does ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml