Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-18
... complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes : The absence of a com- plementary strand eliminates the possibility of forming nonproductive ...
... complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes : The absence of a com- plementary strand eliminates the possibility of forming nonproductive ...
Page 10-19
... complementary radiolabeled DNA ( see Figure 10.1 , page 10.4 ) . Most commonly , the primer is complementary to a region of the lac gene immediately 3 ' to the polycloning site in the mp series of bacteriophage M13 vectors ( Yanisch ...
... complementary radiolabeled DNA ( see Figure 10.1 , page 10.4 ) . Most commonly , the primer is complementary to a region of the lac gene immediately 3 ' to the polycloning site in the mp series of bacteriophage M13 vectors ( Yanisch ...
Page 11-41
... complementary sequences at their 3 ' termini ( Ullrich et al . 1984a ) ( see Figure 11.2 ) . Both strands are extended , resulting in a duplex that may be considerably longer than either of the two original oligonucleotides ( see pages ...
... complementary sequences at their 3 ' termini ( Ullrich et al . 1984a ) ( see Figure 11.2 ) . Both strands are extended , resulting in a duplex that may be considerably longer than either of the two original oligonucleotides ( see pages ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml