Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 92
... complete sequence of the human genome . Although this rapid rate of progress has been catalyzed by many theoretical and technical advances , none has been so informative as the ability to construct libraries of eukaryotic genomic DNA ...
... complete sequence of the human genome . Although this rapid rate of progress has been catalyzed by many theoretical and technical advances , none has been so informative as the ability to construct libraries of eukaryotic genomic DNA ...
Page 13-18
... complete the project The amount of work required to complete a sequencing project can be estimated from the following guidelines : 1. An average of 300-400 nucleotides of sequence can routinely be obtained from a single reaction set . 2 ...
... complete the project The amount of work required to complete a sequencing project can be estimated from the following guidelines : 1. An average of 300-400 nucleotides of sequence can routinely be obtained from a single reaction set . 2 ...
Page 15-50
... complete , stain the gel for 1 hour in 1x TAE containing ethidium bromide ( 0.5 μg / ml ) ( see Chapter 6 , pages 6.15- 6.17 ) . 4. Determine the amount of topoisomerase I that is required for complete conversion of the control plasmid ...
... complete , stain the gel for 1 hour in 1x TAE containing ethidium bromide ( 0.5 μg / ml ) ( see Chapter 6 , pages 6.15- 6.17 ) . 4. Determine the amount of topoisomerase I that is required for complete conversion of the control plasmid ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml