Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-49
... concentration of 500 μg / ml in hybridization solutions containing dextran sulfate ; in hybridization solutions that do not contain dextran sulfate , heparin is used at a concentration of 50 μg / ml ( Singh and Jones , 1984 ) ...
... concentration of 500 μg / ml in hybridization solutions containing dextran sulfate ; in hybridization solutions that do not contain dextran sulfate , heparin is used at a concentration of 50 μg / ml ( Singh and Jones , 1984 ) ...
Page 10-20
... concentration in excess of K and one radiolabeled dNTP at a lower concentration , which is usually below Km Under these conditions , the proportion of radiolabel that is incorporated into DNA is very high , even though the rate of the ...
... concentration in excess of K and one radiolabeled dNTP at a lower concentration , which is usually below Km Under these conditions , the proportion of radiolabel that is incorporated into DNA is very high , even though the rate of the ...
Page 10-32
... concentration . More than 90 % of the transcripts are full - length when the concentration of each of the rNTPs is 50 μм or greater . Because bacteriophage SP6 DNA - dependent RNA polymerase is more tolerant of lower concentrations of ...
... concentration . More than 90 % of the transcripts are full - length when the concentration of each of the rNTPs is 50 μм or greater . Because bacteriophage SP6 DNA - dependent RNA polymerase is more tolerant of lower concentrations of ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml