Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 81
Page xxi
... Constructed in Plasmids 12.8 Expression Libraries Constructed in Bacteriophage λ 12.10 Using Antibodies in Immunological Screening 12.11 Choosing the Antibody 12.11 Purification of Antisera 12.13 Methods Used to Detect Antibodies Bound ...
... Constructed in Plasmids 12.8 Expression Libraries Constructed in Bacteriophage λ 12.10 Using Antibodies in Immunological Screening 12.11 Choosing the Antibody 12.11 Purification of Antisera 12.13 Methods Used to Detect Antibodies Bound ...
Page 8-38
... constructed in Agt11 , like those constructed in Agt10 , can be screened with nucleic acid probes . However , libraries constructed in Agt10 are far better suited for this purpose . An efficient method is available to select against ...
... constructed in Agt11 , like those constructed in Agt10 , can be screened with nucleic acid probes . However , libraries constructed in Agt10 are far better suited for this purpose . An efficient method is available to select against ...
Page 12-10
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Expression Libraries Constructed in Bacteriophage λ The vast majority of cDNA libraries constructed in bacteriophage λ expres- sion vectors use Agt11 ( or its close relatives Agt18-23 ) ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Expression Libraries Constructed in Bacteriophage λ The vast majority of cDNA libraries constructed in bacteriophage λ expres- sion vectors use Agt11 ( or its close relatives Agt18-23 ) ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml