Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 65
Page 10-24
... desired hotness to the adhesive labels . Attach radioactive- warning tape to the pen , and store it in an ... desired fragment of DNA . Use the hole as a template to mark the position of the DNA on the Saran Wrap covering the gel ...
... desired hotness to the adhesive labels . Attach radioactive- warning tape to the pen , and store it in an ... desired fragment of DNA . Use the hole as a template to mark the position of the DNA on the Saran Wrap covering the gel ...
Page 15-30
... desired , a phosphorylated linker can be inserted at the site of recircularization by including the linker during ... desired size range ) , pick a large number of individual transformed colonies or plaques and determine the size of the ...
... desired , a phosphorylated linker can be inserted at the site of recircularization by including the linker during ... desired size range ) , pick a large number of individual transformed colonies or plaques and determine the size of the ...
Page 15-52
... desired mutation by oligonucleotide hybridization ( Wallace et al . 1979 , 1981b ) , ( 4 ) the development of a versatile set of bacteriophage M13 vectors ( for review , see Chapter 4 ) , and ( 5 ) the invention of genetic methods to ...
... desired mutation by oligonucleotide hybridization ( Wallace et al . 1979 , 1981b ) , ( 4 ) the development of a versatile set of bacteriophage M13 vectors ( for review , see Chapter 4 ) , and ( 5 ) the invention of genetic methods to ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml