Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 86
Page 8-50
... detected histochemically ( e.g. , alkaline phosphatase ) . The key to success with this method lies in the quality of the antibody . It is essential that the antibody efficiently recognize the denatured protein ( i.e. , it should ...
... detected histochemically ( e.g. , alkaline phosphatase ) . The key to success with this method lies in the quality of the antibody . It is essential that the antibody efficiently recognize the denatured protein ( i.e. , it should ...
Page 9-31
... detectable hybridization signal depends on a number of factors , including the proportion of the genome that is ... detected in an overnight exposure if 10 μg of genomic DNA is transferred to the filter and hybridized to a probe ...
... detectable hybridization signal depends on a number of factors , including the proportion of the genome that is ... detected in an overnight exposure if 10 μg of genomic DNA is transferred to the filter and hybridized to a probe ...
Page 9-56
... detect single - copy sequences in restriction digests of eukaryotic genomic DNA that have been transferred to solid supports . As discussed in Chapter 11 , hybrids of this length are stable enough to be detected in practice only if they ...
... detect single - copy sequences in restriction digests of eukaryotic genomic DNA that have been transferred to solid supports . As discussed in Chapter 11 , hybrids of this length are stable enough to be detected in practice only if they ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml