Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 92
Page 9-24
... digestion of linkers ) to generate cohesive termini compatible with those of the vectors used to generate genomic DNA libraries ( Maniatis et al . 1978 ) . On the other hand , partial digestion with restriction enzymes that recognize ...
... digestion of linkers ) to generate cohesive termini compatible with those of the vectors used to generate genomic DNA libraries ( Maniatis et al . 1978 ) . On the other hand , partial digestion with restriction enzymes that recognize ...
Page 9-32
... digestion of high - molecular - weight DNA is unevenness of digestion caused by variations in the local concentrations of DNA . Clumps of DNA are relatively inaccessible to restriction enzymes and can be digested only from the outside ...
... digestion of high - molecular - weight DNA is unevenness of digestion caused by variations in the local concentrations of DNA . Clumps of DNA are relatively inaccessible to restriction enzymes and can be digested only from the outside ...
Page 13-21
... digestion with restriction en- zymes , sonication , and digestion with pancreatic DNAase I in the presence of Mn . Sonication and digestion with DNAase I are superior to digestion with restriction enzymes in a number respects . First ...
... digestion with restriction en- zymes , sonication , and digestion with pancreatic DNAase I in the presence of Mn . Sonication and digestion with DNAase I are superior to digestion with restriction enzymes in a number respects . First ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml