Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 41
Page 8-62
... dithiothreitol 2.0 με 25 units RNAase inhibitor H2O to a final volume of 48 μl Add 40 units of avian reverse transcriptase and mix as described above . Incubate for 2 hours at 42 ° C . Preparations of reverse transcriptase purified from ...
... dithiothreitol 2.0 με 25 units RNAase inhibitor H2O to a final volume of 48 μl Add 40 units of avian reverse transcriptase and mix as described above . Incubate for 2 hours at 42 ° C . Preparations of reverse transcriptase purified from ...
Page 10-7
... dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) Divide the stock solution into small aliquots and store them at -20 ° C . 10 × Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 100 mm dithiothreitol 500 μg ...
... dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) Divide the stock solution into small aliquots and store them at -20 ° C . 10 × Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 100 mm dithiothreitol 500 μg ...
Page 12-34
... dithiothreitol to a final concentration of 1 mM . F 10 × Binding buffer 250 mM HEPES ( pH 7.9 ) 30 mM MgCl2 , 40 mM KCl 5. Decant the second batch of denaturation solution into a graduated cylin- der . Dilute the solution with an equal ...
... dithiothreitol to a final concentration of 1 mM . F 10 × Binding buffer 250 mM HEPES ( pH 7.9 ) 30 mM MgCl2 , 40 mM KCl 5. Decant the second batch of denaturation solution into a graduated cylin- der . Dilute the solution with an equal ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml