Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-18
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
Page 14-28
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
Page 15-20
... double- stranded DNA is given by the equation : dM -2VM , t = m n dt [ Km + ( S ) , ] n where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , Vm = maximum reaction velocity ( expressed as moles of nu ...
... double- stranded DNA is given by the equation : dM -2VM , t = m n dt [ Km + ( S ) , ] n where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , Vm = maximum reaction velocity ( expressed as moles of nu ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml